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细胞空白比空白质控球荧光高怎么办?

在用流式进行细胞表面蛋白的定量时,常会发现空白微球的荧光比细胞的荧光值低,这时该怎么办(计算)?
The blank bead is included as a convenience to the client to determine the lower detection threshold of the instrument at particular settings. It is not necessary in most cases for clients to use the blank - and the blank does not impact the results calculated by QuickCal (you can have them test this by zeroing out the blank value, or changing it to various random values). What IS important is that the client take the blank cell value and subtract the QuickCal calculated ABC value from the cell-stained QuickCal calculated. 

For example. The Cell Blank may have a FACS Median of 400 channels - which Quickcal calculates as an ABC of 1,000. The Cell Stained may have a FACS Median of 10,000 channels, which quickcal calculates as an ABC of 25,000. The client should take the Stained - Blank to get the corrected ABC value for the stained, which in this example would be 24,000 ABC (25,‪000 - 1‬,000). 

ABC stands for Antibody Binding Capacity. 

So in short - no problems are occurring here. They can continue to use the blank bead to establish the lower detection threshold (but it’s not terribly important), most important is to subtract the blank cell ABC from the stained cell ABC in Quickcal. 

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